LOCUS       40924_48633       12799 bp DNA     circular SYN 18-DEC-2018
DEFINITION  BAC cloning vector P[acman]-ApR-F-2-5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Venken KJ, He Y, Hoskins RA, Bellen HJ.
  TITLE     P[acman]: a BAC transgenic platform for targeted insertion of large 
            DNA fragments in D. melanogaster
  JOURNAL   Science 314 (5806), 1747-1751 (2006)
  PUBMED    17138868
REFERENCE   2  (bases 1 to 12799)
  AUTHORS   Venken KJT., He Y, Hoskins RA, Bellen HJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2006) Molecular and Human Genetics, Baylor College
            of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 12799)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12799)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2006"; volume: "314"; issue: "5806"; pages: "1747-1751"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-NOV-2006) Molecular and Human Genetics, Baylor College of 
            Medicine, One Baylor Plaza, Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(244..717)
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(793..1761)
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1764..2936)
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    complement(3262..3512)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3518..4270)
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      complement(4361..4580)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4659..5372
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6236..6338
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        6339..6410
                     /label=AmpR promoter
     CDS             6411..7268
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(7692..7924)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     7931..7947
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7957..7975
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8019..8093
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(8112..8130)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    8166..12181
                     /label=white dominant eye marker
                     /note="white dominant eye marker"
     CDS             join(8650..8721,9123..9396,9471..10125,10187..10502,
                     10706..10837,10908..11522)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    complement(12182..12767)
                     /label=P element 5' end
ORIGIN
        1 tcgacagcga cacacttgca tcggatgcag cccggttaac gtgccggcac ggcctgggta
       61 accaggtatt ttgtccacat aaccgtgcgc aaaatgttgt ggataagcag gacacagcag
      121 caatccacag caggcataca accgcacacc gaggttactc cgttctacag gttacgacga
      181 catgtcaata cttgcccttg acaggcattg atggaatcgt agtctcacgc tgatagtctg
      241 atcgacaata caagtgggac cgtggtccca gaccgataat cagaccgaca acacgagtgg
      301 gatcgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact
      361 aataatcaga ccgacgatac gagtgggacc gtggttccag actaataatc agaccgacga
      421 tacgagtggg accgtggtcc cagactaata atcagaccga cgatacgagt gggaccatgg
      481 tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccagt ctgattatca
      541 gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg
      601 ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagtc
      661 tgattatcag accgacgata caagtggaac agtgggccca gagagaatat tcaggccagt
      721 tatgctttct ggcctgtaac aaaggacatt aagtaaagac agataaacgt agactaaaac
      781 gtggtcgcat cagggtgctg gcttttcaag ttccttaaga atggcctcaa ttttctctat
      841 acactcagtt ggaacacgag acctgtccag gttaagcacc attttatcgc ccttatacaa
      901 tactgtcgct ccaggagcaa actgatgtcg tgagcttaaa ctagttcttg atgcagatga
      961 cgttttaagc acagaagtta aaagagtgat aacttcttca gcttcaaata tcaccccagc
     1021 ttttttctgc tcatgaaggt tagatgcctg ctgcttaagt aattcctctt tatctgtaaa
     1081 ggctttttga agtgcatcac ctgaccgggc agatagttca ccggggtgag aaaaaagagc
     1141 aacaactgat ttaggcaatt tggcggtgtt gatacagcgg gtaataatct tacgtgaaat
     1201 attttccgca tcagccagcg cagaaatatt tccagcaaat tcattctgca atcggcttgc
     1261 ataacgctga ccacgttcat aagcacttgt tgggcgataa tcgttaccca atctggataa
     1321 tgcagccatc tgctcatcat ccagctcgcc aaccagaaca cgataatcac tttcggtaag
     1381 tgcagcagct ttacgacggc gactcccatc ggcaatttct atgacaccag atactcttcg
     1441 accgaacgcc ggtgtctgtt gaccagtcag tagaaaagaa gggatgagat catccagtgc
     1501 gtcctcagta agcagctcct ggtcacgttc attacctgac catacccgag aggtcttctc
     1561 aacactatca ccccggagca cttcaagagt aaacttcaca tcccgaccac atacaggcaa
     1621 agtaatggca ttaccgcgag ccattactcc tacgcgcgca attaacgaat ccaccatcgg
     1681 ggcagctggt gtcgataacg aagtatcttc aaccggttga gtattgagcg tatgttttgg
     1741 aataacaggc gcacgcttca ttatctaatc tcccagcgtg gtttaatcag acgatcgaaa
     1801 atttcattgc agacaggttc ccaaatagaa agagcatttc tccaggcacc agttgaagag
     1861 cgttgatcaa tggcctgttc aaaaacagtt ctcatccgga tctgaccttt accaacttca
     1921 tccgtttcac gtacaacatt ttttagaacc atgcttcccc aggcatcccg aatttgctcc
     1981 tccatccacg gggactgaga gccattacta ttgctgtatt tggtaagcaa aatacgtaca
     2041 tcaggctcga accctttaag atcaacgttc ttgagcagat cacgaagcat atcgaaaaac
     2101 tgcagtgcgg aggtgtagtc aaacaactca gcaggcgtgg gaacaatcag cacatcagca
     2161 gcacatacga cattaatcgt gccgataccc aggttaggcg cgctgtcaat aactatgaca
     2221 tcatagtcat gagcaacagt ttcaatggcc agtcggagca tcaggtgtgg atcggtgggc
     2281 agtttacctt catcaaattt gcccattaac tcagtttcaa tacggtgcag agccagacag
     2341 gaaggaataa tgtcaagccc cggccagcaa gtgggcttta ttgcataagt gacatcgtcc
     2401 ttttccccaa gatagaaagg caggagagtg tcttctgcat gaatatgaag atctggtacc
     2461 catccgtgat acattgaggc tgttccctgg gggtcgttac cttccacgag caaaacacgt
     2521 agccccttca gagccagatc ctgagcaaga tgaacagaaa ctgaggtttt gtaaacgcca
     2581 cctttatggg cagcaacccc gatcaccggt ggaaatacgt cttcagcacg tcgcaatcgc
     2641 gtaccaaaca catcacgcat atgattaatt tgttcaattg tataaccaac acgttgctca
     2701 acccgtcctc gaatttccat atccgggtgc ggtagtcgcc ctgctttctc ggcatctctg
     2761 atagcctgag aagaaacccc aactaaatcc gctgcttcac ctattctcca gcgccgggtt
     2821 attttcctcg cttccgggct gtcatcatta aactgtgcaa tggcgatagc cttcgtcatt
     2881 tcatgaccag cgtttatgca ctggttaagt gtttccatga gtttcattct gaacatcctt
     2941 taatcattgc tttgcgtttt tttattaaat cttgcaattt actgcaaagc aacaacaaaa
     3001 tcgcaaagtc atcaaaaaac cgcaaagttg tttaaaataa gagcaacact acaaaaggag
     3061 ataagaagag cacatacctc agtcacttat tatcactagc gctcgccgca gccgtgtaac
     3121 cgagcatagc gagcgaactg gcgaggaagc aaagaagaac tgttctgtca gatagctctt
     3181 acgctcagcg caagaagaaa tatccaccgt gggaaaaact ccaggtagag gtacacacgc
     3241 ggatagccaa ttcagagtaa taaactgtga taatcaaccc tcatcaatga tgacgaacta
     3301 acccccgata tcaggtcaca tgacgaaggg aaagagaagg aaatcaactg tgacaaactg
     3361 ccctcaaatt tggcttcctt aaaaattaca gttcaaaaag tatgagaaaa tccatgcagg
     3421 ctgaaggaaa cagcaaaact gtgacaaatt accctcagta ggtcagaaca aatgtgacga
     3481 accaccctca aatctgtgac agataaccct cagactatcc tgtcgtcatg gaagtgatat
     3541 cgcggaagga aaatacgata tgagtcgtct ggcggccttt ctttttctca atgtatgaga
     3601 ggcgcattgg agttctgctg ttgatctcat taacacagac ctgcaggaag cggcggcgga
     3661 agtcaggcat acgctggtaa ctttgaggca gctggtaacg ctctatgatc cagtcgattt
     3721 tcagagagac gatgcctgag ccatccggct tacgatactg acacagggat tcgtataaac
     3781 gcatggcata cggattggtg atttcttttg tttcactaag ccgaaactgc gtaaaccggt
     3841 tctgtaaccc gataaagaag ggaatgagat atgggttgat atgtacactg taaagccctc
     3901 tggatggact gtgcgcacgt ttgataaacc aaggaaaaga ttcatagcct ttttcatcgc
     3961 cggcatcctc ttcagggcga taaaaaacca cttccttccc cgcgaaactc ttcaatgcct
     4021 gccgtatatc cttactggct tccgcagagg tcaatccgaa tatttcagca tatttagcaa
     4081 catggatctc gcagataccg tcatgttcct gtagggtgcc atcagatttt ctgatctggt
     4141 caacgaacag atacagcata cgtttttgat cccgggagag actatatgcc gcctcagtga
     4201 ggtcgtttga ctggacgatt cgcgggctat ttttacgttt cttgtgattg ataaccgctg
     4261 tttccgccat gacagatcca tgtgaagtgt gacaagtttt tagattgtca cactaaataa
     4321 aaaagagtca ataagcaggg ataactttgt gaaaaaacag cttcttctga gggcaatttg
     4381 tcacagggtt aagggcaatt tgtcacagac aggactgtca tttgagggtg atttgtcaca
     4441 ctgaaagggc aatttgtcac aacaccttct ctagaaccag catggataaa ggcctacaag
     4501 gcgctctaaa aaagaagatc taaaaactat aaaaaaaata attataaaaa tatccccgtg
     4561 gataagtgga taaccccaag ggaagttttt tcaggcatcg tgtgtaagca gaatatataa
     4621 gtgctgttcc ctggtgcttc ctcgctcact cgaaattccc ctttccgacg ctcaccgggc
     4681 tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc
     4741 cgtcgaagcc gtgtgcgaga caccgcggcc ggccgccggc gttgtggata cctcgcggaa
     4801 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
     4861 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
     4921 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
     4981 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
     5041 ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg
     5101 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
     5161 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
     5221 tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct
     5281 tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc
     5341 ggccgcgaac ggcctcaccc caaaaatggc aggggatctc gagggcttcg ccctgtcgct
     5401 cgactgcggc gagcactact ggctgtaaaa ggacagacca catcatggtt ctgtgttcat
     5461 taggttgttc tgtccattgc tgacataatc cgctccactt caacgtaaca ccgcacgaag
     5521 atttctattg ttcctgaagg catattcaaa tcgttttcgt taccgcttgc aggcatcatg
     5581 acagaacact acttcctata aacgctacac aggctcctga gattaataat gcggatctct
     5641 acgataatgg gagattttcc cgactgtttc gttcgcttct cagtggataa cagccagctt
     5701 ctctgtttaa cagacaaaaa cagcatatcc actcagttcc acatttccat ataaaggcca
     5761 aggcatttat tctcaggata attgtttcag catcgcaacc gcatcagact ccggcatcgc
     5821 aaactgcacc cggtgccggg cagccacatc cagcgcaaaa accttcgtgt agacttccgt
     5881 tgaactgatg gacttatgtc ccatcaggct ttgcagaact ttcagcggta taccggcata
     5941 cagcatgtgc atcgcatagg aatggcggaa cgtatgtggt gtgaccggaa cagagaacgt
     6001 cacaccgtca gcagcagcgg cggcaaccgc ctccccaatc caggtcctga ccgttctgtc
     6061 cgtcacttcc cagatccgcg ctttctctgt ccttcctgtg cgacggttac gccgctccat
     6121 gagcttatcg cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg
     6181 gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc
     6241 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     6301 ttgagttatc gagattttca ggagctaagg aagctaaaca ttcaaatatg tatccgctca
     6361 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6421 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6481 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6541 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6601 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6661 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6721 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6781 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6841 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6901 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6961 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7021 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7081 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7141 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7201 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7261 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7321 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7501 cagcggtggt ttgtttgccg gatcaagagc taccaactct tttttttaag gcagttattg
     7561 gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag
     7621 aaattcgatg ataagctgtc aaacatgaga attggtcgag atccgctagc tcacgatgag
     7681 aatggccaga ccatgatgaa ataacataag gtggtcccgt cggcaagaga catccactta
     7741 acgtatgctt gcaataagtg cgagtgaaag gaatagtatt ctgagtgtcg tattgagtct
     7801 gagtgagaca gcgatatgat tgttgattaa cccttagcat gtccgtgggg tttgaattaa
     7861 ctcataatat taattagacg aaattatttt taaagtttta tttttaataa tttgcgagta
     7921 cgcaaccggt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
     7981 ttggagctcg tttaaacctc gagcggtccg ttatcgatgg cgcgccggcc ttaatggccg
     8041 cggccgcatt taaatgtcga cttaattaag gccggccgcg atcgcgcccg ggcggtaccc
     8101 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     8161 tttccgaatt ctagtatgta tgtaagttaa taaaacccat ttttgcggaa agtagataaa
     8221 aaaaacattt ttttttttta ctgcactgga tatcattgaa cttatctgat cagttttaaa
     8281 tttacttcga tccaagggta tttgatgtac caggttcttt cgattacctc tcactcaaaa
     8341 tgacattcca ctcaaagtca gcgctgtttg cctccttctc tgtccacaga aatatcgccg
     8401 tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt acgtagcgtc
     8461 aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca agcggtttac
     8521 gccatcaatt aaacacaaag tgctgtgcca aaactcctct cgcttcttat ttttgtttgt
     8581 tttttgagtg attggggtgg tgattggttt tgggtgggta agcaggggaa agtgtgaaaa
     8641 atcccggcaa tgggccaaga ggatcaggag ctattaattc gcggaggcag caaacaccca
     8701 tctgccgagc atctgaacaa tgtgagtagt acatgtgcat acatcttaag ttcacttgat
     8761 ctataggaac tgcgattgca acatcaaatt gtctgcggcg tgagaactgc gacccacaaa
     8821 aatcccaaac cgcaattgca caaacaaata gtgacacgaa acagattatt ctggtagctg
     8881 ttctcgctat ataagacaat ttttgagatc atatcatgat caagacatct aaaggcattc
     8941 attttcgact atattctttt ttacaaaaaa tataacaacc agatatttta agctgatcct
     9001 agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc ggggtacttt
     9061 ttgttcgggg ttagatgagc ataacgcttg tagttgatat ttgagatccc ctatcattgc
     9121 agggtgacag cggagcggct tcgcagagct gcattaacca gggcttcggg caggccaaaa
     9181 actacggcac gctccggcca cccagtccgc cggaggactc cggttcaggg agcggccaac
     9241 tagccgagaa cctcacctat gcctggcaca atatggacat ctttggggcg gtcaatcagc
     9301 cgggctccgg atggcggcag ctggtcaacc ggacacgcgg actattctgc aacgagcgac
     9361 acataccggc gcccaggaaa catttgctca agaacggtga gtttctattc gcagtcggct
     9421 gatctgtgtg aaatcttaat aaagggtcca attaccaatt tgaaactcag tttgcggcgt
     9481 ggcctatccg ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa agacgaccct
     9541 gctgaatgcc cttgcctttc gatcgccgca gggcatccaa gtatcgccat ccgggatgcg
     9601 actgctcaat ggccaacctg tggacgccaa ggagatgcag gccaggtgcg cctatgtcca
     9661 gcaggatgac ctctttatcg gctccctaac ggccagggaa cacctgattt tccaagccat
     9721 ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat
     9781 ccaggagctt tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa
     9841 aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc tccgaggctc taaccgatcc
     9901 gccgcttctg atctgcgatg agcccacctc cggactggac tcctttaccg cccacagcgt
     9961 cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga ccattcatca
    10021 gccgtcttcc gagctgtttg agctctttga caagatcctt ctgatggccg agggcagggt
    10081 agctttcttg ggcactccca gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt
    10141 ttattaaggg tatctagtat tacataacat ctcaactcct atccagcgtg ggtgcccagt
    10201 gtcctaccaa ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac
    10261 gggagatcga gtcccgtgat cggatcgcca agatatgcga caattttgcc attagcaaag
    10321 tagcccggga tatggagcag ttgttggcca ccaaaaatct ggagaagcca ctggagcagc
    10381 cggagaatgg gtacacctac aaggccacct ggttcatgca gttccgggcg gtcctgtggc
    10441 gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt attcagacaa
    10501 cggtgagtgg ttccagtgga aacaaatgat ataacgctta caattcttgg aaacaaattc
    10561 gctagatttt agatagaatt gcctgattcc acacccttct tagttttttt caatgagatg
    10621 tatagtttat agttttgcag aagataaata aatttcattt aactcgcgaa tattaatgag
    10681 atgcgagtaa cattttaatt tgcagatggt tgccatcttg attggcctca tctttttggg
    10741 ccaacaactc acgcaagtgg gtgtgatgaa tatcaacgga gccatcttcc tcttcctgac
    10801 caacatgacc tttcaaaacg tctttgccac gataaatgta agtcatgttt agaatacatt
    10861 tgcatttcaa taatttacta actttctaat gaatcgattc gatttaggtg ttcacctcag
    10921 agctgccagt ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac acatactttc
    10981 tgggcaaaac gattgccgaa ttgccgcttt ttctcacagt gccactggtc ttcacggcga
    11041 ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc
    11101 tggtcactct ggtggccaat gtgtcaacgt ccttcggata tctaatatcc tgcgccagct
    11161 cctcgacctc gatggcgctg tctgtgggtc cgccggttat cataccattc ctgctctttg
    11221 gcggcttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct
    11281 catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg
    11341 gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg
    11401 agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg gccattctca
    11461 tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt
    11521 agccgacata tatccgaaat aactgcttgt tttttttttt taccattatt accatcgtgt
    11581 ttactgttta ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag
    11641 atatgaccta tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat
    11701 ttgtcttcta accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg
    11761 ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt
    11821 cataattcac ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct
    11881 ctccgcaaca cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc
    11941 gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa attagtaata
    12001 aatatttgta tgtacaattt atttgctcca atatatttgt atatatttcc ctcacagcta
    12061 tatttattct aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt
    12121 gattagcgtt acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca
    12181 tgctgctgct ctaaacgacg catttcgtac tccaaagtac gaattttttc cctcaagctc
    12241 ttattttcat taaacaatga acaggaccta acgcacagtc acgttattgt ttacataaat
    12301 gatttttttt actattcaaa cttactctgt ttgtgtactc ccactggtat agccttcttt
    12361 tatcttttct ggttcaggct ctatcacttt actaggtacg gcatctgcgt tgagtcgcct
    12421 ccttttaaat gtctgacctt ttgcaggtgc agccttccac tgcgaatcat taaagtgggt
    12481 atcacaaatt tgggagtttt caccaaggct gcacccaagg ctctgctccc acaattttct
    12541 cttaatagca cacttcggca cgtgaattaa ttttactcca gtcacagctt tgcagcaaaa
    12601 tttgcaatat ttcatttttt tttattccac gtaagggtta atgttttcaa aaaaaaattc
    12661 gtccgcacac aacctttcct ctcaacaagc aaacgtgcac tgaatttaag tgtatacttc
    12721 ggtaagcttc ggctatcgac gggaccacct tatgttattt catcatgggc cagacccacg
    12781 tagtccagac tagtggatc
//
